Research Directions and labs
Research
Biotechnology and structural biology

A. Dishlers lab

Andris Dišlers , Dr. biol

Senior researcher
Phone: +371 67808213
E-mail: dishlers@biomed.lu.lv

Research

Basically, our group is working on projects developing recombinant vaccine candidates based on VLPs (Virus-Like Particles). Expression systems (mostly E.coli) and purification procedures for large spectrum of different proteins, VLPs and their chimeric derivatives are systematically developed and different expression-purification conditions are compared for the best yield and outcome of particular target proteins and vaccine candidates. Also, relevant functional proteins are expressed and purified to the level suitable for chemical coupling to VLPs of RNA-phages and hepatitis B virus (HBV) core-antigen (HBcAg). Genetically modified chimeric VLPs displaying immunologically relevant epitopes of HBV, HCV, Rubella and other viruses are systematically investigated and characterized for stability on subunit and VLP level, and their immunological potential in mouse models is evaluated. For T-cell immunogenicity of VLP-based vaccine candidates, it is crucial to have the characterized nucleic acid material inside the capsids, therefore, HBc-VLPs of different structure (with variable nucleic acid binding capacity) and origin (using HBcAg from different HBV genotypes) are investigated in our group for packaging of immunomodulatory substances such as oligonucleotides, single- and double-stranded RNA for their T-cell inducing potential and biotechnological properties. To reach the goal, VLP purification protocols of extra-pure quality are developed for HBc antigen itself and its structural derivatives, based on dissociation of HBc VLPs to dimeric subunits with further reassociation in the presence or absence of target substances (nucleic acids, peptides, proteins), - in latter case, the VLPs are loaded with substances after their reconstruction from dimers. Ptrp based system, pET, pQE and pCold expression systems have been used in our facility to reach the goal – high level expression and yield of target proteins. Advanced protein purification systems are used for purification of VLPs and other target proteins.

Scientific Staff

Ivars Petrovskis , Dr. biol, ivars@biomed.lu.lv

Ivars Petrovskis , Dr. biol

Senior researcher
Phone: +371 67808213
E-mail: ivars@biomed.lu.lv

Indulis Cielēns , Dr. biol., cielens@biomed.lu.lv

Indulis Cielēns , Dr. biol.

Senior researcher
Phone: +371 67808004
E-mail: cielens@biomed.lu.lv

Jānis Bogans , MSc chem., janis@biomed.lu.lv

Jānis Bogans , MSc chem.

Researcher
Phone: +371 67808200
E-mail: janis@biomed.lu.lv

Ilva Liekniņa , MSc.biol, ilva@biomed.lu.lv

Ilva Liekniņa , MSc.biol

Research assistants
Phone: +371 67808213
E-mail: ilva@biomed.lu.lv

Ināra Akopjana , in.ak@inbox.lv

Ināra Akopjana

Scientific assistant
Phone: 67808213
E-mail: in.ak@inbox.lv

Jeļena Žakova , MSc. biol., jelena@biomed.lu.lv

Jeļena Žakova , MSc. biol.

Research assistants
Phone: +371 67808213
E-mail: jelena@biomed.lu.lv

Guntars Zariņš , MSc. biol., guntarsza@inbox.lv

Guntars Zariņš , MSc. biol.

Laboratory assistant
Phone: +371 67808213
E-mail: guntarsza@inbox.lv

Sarmīte Akopjana , sarmite.kup@inbox.lv

Sarmīte Akopjana

Laboratory assistant
Phone: +371 67808213
E-mail: sarmite.kup@inbox.lv

Cooperation partners

Publications


All publications

Quick links

Group quick links



Mājas lapas izstrādi finansēja ERAF 2.1.1.2. aktivitātes projekts Nr. 2010/0196/2DP/2.1.1.2.0/10/APIA/VIAA/004 "Latvijas biomedicīnas pētījumu integrācija Eiropas zinātnes telpā".