Research Facilities and services Cell biology core facility
Facilities and services

Cell biology core facility

Human and animal cell culturing has become an integrated part of biomedical and biotechnology research. The Cell biology core facility is the part of Biomedical technology complex at BMC that joins the knowledge, skills and expertise of research personnel as well as gives centralised access to broad array of equipment. The core facility ensures mammalian cell culture maintenance, characterisation and functional testing and the obtaining of primary cell cultures. 

The cell culturing area is designed and equipped according to the biosafety level 2. There are three separated blocks for the work with (1) certified cell lines (i.e., obtained from ATCC, ECACC etc.), (2) clinical material to obtain primary cell cultures/lines, and (3) cell lines infected with non-pathogenic viruses or virus-like particles. The main cell culture area is accompanied with a support room for washing and sterilisation, several additional microscopy rooms and a biochemistry room. The facility ensures short-term and long-term storage of cells at -80oC and liquid nitrogen tanks.

Provided services:

  • Cell culturing
  • Obtaining of primary cell cultures/lines
  • Cell line bank & cell culture storage
  • Routine testing for mycoplasma contamination
  • Cell counting & sorting (FACSAria, BD)
  • Fluorescent microscopy (LEICA DMI 3000 B, LEICA DM 3000 and LEICA TCS SP2 SE)
  • ELISPOT analyses (ImmunoSpot CTL)
  • Biochemistry room for cell & tissue sample characterisation
  • Student training for cell culturing & testing

 

The core facility has the capacity of six working places within the cell culture rooms, three at fluorescent microscopes; biochemistry room holds a place for up to five people. Under the supervision of qualified specialists, personnel training for golden standards of cell culturing and characterisation is ensured; special attention is paid to the student training. To ensure optimal use of the equipment, it is operated by designated BMC personnel. 

Responsible personnel:

Ramona Petrovska , MSc. biol.

Head of Cell biology core facility, researcher
Phone: +371 28226298
E-mail: ramona@biomed.lu.lv

 Cell culture bank establishment, student training

Dace Pjanova , Dr. biol.

Senior researcher
Phone: +371 29468992
E-mail: dace@biomed.lu.lv

 Fluorescent microscopy

Lilite Sadovska , MSc. biol.

Research assistants
Phone: +371 28222543
E-mail: lilite@biomed.lu.lv

 FC/FACS

Elīna Zandberga , MSc. biol.

Research assistants
Phone: +371 26529330
E-mail: elina@biomed.lu.lv

 Cell culturing, ELISPOT


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Mājas lapas izstrādi finansēja ERAF 2.1.1.2. aktivitātes projekts Nr. 2010/0196/2DP/2.1.1.2.0/10/APIA/VIAA/004 "Latvijas biomedicīnas pētījumu integrācija Eiropas zinātnes telpā".