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Development of a bacterial microcompartment-based nanoreactor platform for metal-containing nanoparticle synthesis

Funding: European Regional Development Fund (ERDF) “On Implementation of Activity 1.1.1.2 “Post-doctoral Research Aid” of the Specific Aid Objective 1.1.1 “To increase the research and innovative capacity of scientific institutions of Latvia and the ability to attract external financing, investing in human resources and infrastructure” of the Operational Programme “Growth and Employment”

Project Title: Development of a bacterial microcompartment-based nanoreactor platform for metal-containing nanoparticle synthesis 

Project No.: 1.1.1.2/VIAA/4/20/705

Period: 1 January 2021 – 30 June 2023

Project costs: 111 505.00 EUR

Project implementer: Ph.D. Gints Kalniņš

 

The aim of the project is to develop a bacterial microcompartment-based nanoreactor platform for metal-containing nanoparticle synthesis. Bacterial microcompartments are bacterial organelles consisting of a protein shell encapsulating enzymes performing concerted functions. The aim of this project is to redesign the shell of bacterial microcompartments for non-native enzyme and metal-reducing peptide encapsulation, and use it for metal-containing nanoparticle synthesis. Such a platform could have future aplications in such fields as nanotechnology, medicine or envirionmental engeneering. This project will consist of adaptation of bacterial microcompartment shell for encapsulation of enzymes and peptides necessary for metal containing nanoparticle synthesis; construction, production and purification of such nanoreactor candidates, testing of various nanoparticle synthesis conditions and characterization of the obtained newly synthesized products.

Information published 04.01.2021.

 

Project progress

 1 January 2021 – 31 March 2021

Successfully performed negative staining transmission electronmicroscopy analysis of Klebsiella pneumoniae cell slices and confirmed observation of GRM2 bacterial microcompartment shell substructures. Started preliminary experiments for cryo-electrontomography analysis in cooperation with CEITEC institute. Performed cryo electronmicroscopy analysis of larger-sized GRM2 bacterial microcompartment particles. Started construction of expression vectors for production of bacterial microcompartment shells containing encapsulates enzymes and metal-reducing peptides.

Information published 31.03.2021. 

 

Project progress

 1 April 2021 – 30 June 2021

Published one publication in scientific journal about diversity of bacterial microcompartment shells. Presented popular scientific presentation about bacterial microcompartments during Researchers' night. Continued work on construction of expression vectors for production of bacterial microcompartment shells containing encapsulates enzymes and metal-reducing peptides.

Information published 30.06.2021. 



Mājas lapas izstrādi finansēja ERAF 2.1.1.2. aktivitātes projekts Nr. 2010/0196/2DP/2.1.1.2.0/10/APIA/VIAA/004 "Latvijas biomedicīnas pētījumu integrācija Eiropas zinātnes telpā".