Modelling of Hepatitis B virus replication in progenitor hepatocyte-like cells
Project Title: "Modelling of Hepatitis B virus replication in progenitor hepatocyte-like cells"
Funding: LR Ministry of Education and Science and Ministry of Foreign Affairs, programme "Osmose"
Principle Investigator: Dr.biol. A. Zajakina
The aim of the project is the generation of HBV infection model on the basis of hepatic differentiation of nonparenchymal epithelial cells (NPE) isolated from the liver tissue samples. The project has two main parts: (i) the optimization of the protocols, available for the hepatic differentiation of NPE cells (Montpellier); (ii) HBV infection of newly differentiated hepatocyte-like cells (Riga); Human hepatic progenitor cells represent an alternative model of primary human hepatocytes, the source for liver regeneration. Further characterization of conditions allowing the isolation and growth of human adult hepatic progenitor cells and their susceptibility to native liver infections provide a unique information on the potential application of these cells in clinical trials in France. Moreover advanced methods of progenitor cells transfection with specific differentiation factors could be used as modeling differentiation pathways and significantly broaden the application of progenitor cell. The project could open new perspective on the use of NPE as a cellular model to study the physiopathology of HBV infection.