Research Projects All Projects
Projects

Quick links

Structural and kinetic studies of human carbonic anhydrases as multiple drug targets

 

 

Funding: European Regional Development Fund (ERDF) “On Implementation of Activity 1.1.1.2 “Post-doctoral Research Aid” of the Specific Aid Objective 1.1.1 “To increase the research and innovative capacity of scientific institutions of Latvia and the ability to attract external financing, investing in human resources and infrastructure” of the Operational Programme “Growth and Employment”

Project Title: Structural and kinetic studies of human carbonic anhydrases as multiple drug targets

Project No.: 1.1.1.2/VIAA/3/19/464

Period: 36 months (1 February 2020 – 31 January 2023)

Project costs: 133 806.00 EUR

Project implementer: Dr. biol. Jānis Leitāns

 

Summary of project:

Carbonic anhydrases (CAs) are widespread enzymes that catalyze the interconversion of a water molecule and carbon dioxide into bicarbonate and hydrogen ions. There are at least 16 known mammalian CA isoforms. Although most of them are responsible for important processes in the organism, some isoform enzymatic activity is associated with different diseases. Human CA IX (hCA IX) is overexpressed in many tumor types and it has been proposed that hCA IX enzymatic activity favors tumor spread. The project consists of multiple hCA IX-ligand complex structural studies and the acquisition of structural knowledge for mitochondrial CA isoforms Va and Vb. These results will notably increase the available structural data of tumor associated hCA IX and de novo structure determination of hCA Va and/or hCA Vb isoforms with further use in anti-obesity drug design. 

Information published 03.02.2020. 

 

Project progress

 1 February 2020 – 30 April 2020

Started the optimization of the hCA Va and hCA Vb expression, production and purification systems. hCA Va has been successfully expressed in E.coli, resulting in significant amount of enzymatically active and soluble hCA Va protein. Initial purification protocol has been developed with ongoing optimization steps.

Information published 30.04.2020.

 

Project progress

 1 May 2020 – 31 July 2020

Purification system of the hCA Va is being optimized and initial crystallization condition screening was started. hCA IX enzyme was co-crystallized with various sulfonamide inhibitors.

Information published 31.07.2020

 

Project progress

 1 August 2020 – 31 October 2020

hCA IX enzyme was co-crystallized with sulfonamide class inhibitors, collected difraction data and solved 3D structure. Continued screening of the hCA Va crystallization conditions.

Information published 30.10.2020

 

Project progress

 1 November 2020 – 31 January 2021

Optimization of the hCA IX enzyme - benzenesulfonamide derivatives crystallization conditions. Collection of the diffraction data and ligand modeling. hCA Va enzyme crystallization condition optimizing.

Information published 29.01.2021

 

Project progress

 1 February 2021 – 30 April 2021

Continued working on hCA IX enzyme co-crystallization experiments in complex with benzensulfonamides. Further crystallization condition screening for hCA Va.

Information published 30.04.2021

 

Project progress

 1 May 2021 – 31 July 2021

Collected data from hCA IX – ligand complex crystals and solved 3D structure. Continued screening for hCA Va and hVA Vb crystallization conditions.

Information published 30.07.2021

 

Project progress

 1 August 2021 – 31 October 2021

hCA IX was co-crystallized in complex with sulfonamide class inhibitors. Collected diffraction data and solved the 3-D structure.

Information published 29.10.2021



Mājas lapas izstrādi finansēja ERAF 2.1.1.2. aktivitātes projekts Nr. 2010/0196/2DP/2.1.1.2.0/10/APIA/VIAA/004 "Latvijas biomedicīnas pētījumu integrācija Eiropas zinātnes telpā".